Time lapse photography frames showing microvesicle egress from a Timer-CVB3-infected cell.

<p><i>(B–I)</i> Close inspection of image <i>(A)</i> taken at 15 minute intervals in differentiated NPSCs infected with Timer-CVB3 and observed during a six hour span revealed intracellular membrane reorganization (first 8 frames shown). <i>(J)</i> An intracellular microvesicle expressing matured “fluorescent timer” protein and formed following Timer-CVB3 infection was released from the infected cell (frame 9; pink arrow).