Expression of IL-10 and IFN-γ in γδ T cells after i.n. administration of gliadin.

<p>Proportion of IFN-γ (panel A) and IL-10 (panel C) positive γδ T cells within mucosal (NALT, MLN, PLN) and non-mucosal compartments (Spleen, ILN) after i.n. gliadin (white bars) or OVA (black bars) administration in 8-week-old NOD female mice. (B) Example FACS analysis of IFN-γ expression within γδ T cells in MLN and PLN of NOD mice treated i.n. with gliadin or OVA after 4 hours in vitro restimulation with PMA/ionomycin. (C) Example of IFN-γ positivity within γδ T cells of unstimulated controls (PLN) from gliadin-treated NOD mice. Individual measurements were performed on cells pooled from 2–3 experimental animals. Data are expressed as mean values ±SEM, *p<0.05.