Agarose gels showing PCR amplified products from individual chicken hosts and mixtures of DNA sequences from single chicken hosts.

<p>A, Amplified <i>cytb</i> segments from six chickens obtained from two locations: ZZ, Zhangzhou of Fujian province; GZ, Ganzhou of Jiangxi province; B, PCR products of <i>coxIII</i> from four chickens from Ganzhou of Jiangxi province; C, an electropherogram of a small segment of the <i>coxIII</i> from PCR product direct sequencing showing three positions with mixed signals (arrows, A and G); D, polymorphic positions in the <i>coxIII</i> gene segment amplified and sequenced from chicken ZZ24. The positions highlighted in yellow represent positions having two peaks (alleles) in the sequence from PCR product direct sequencing. Samples ZZ24-21 to ZZ24-28 are individual sequences after the PCR products were cloned into TA-vector and sequenced; E, another example as in D from chicken HC2. Ratio, the ratios of minor alleles at each position.