Colonic inflammatory response reconstitution of MAP infected <i>Rag2^−/−</i> mice with CD4⁺CD45RB^lo/int.

<p><b>A.</b> Colonic expression of TNF-α and IL-1β. <b>B</b>. Colonic expression of MMP-9, MMP-13, MMP-14, TIMP-1 and TIMP-2. <b>C</b>. Colonic expression of TLRs. Mice had been infected i.p. with 10⁸ CFU MAP and 4 weeks later reconstituted with CD4⁺CD45RB^lo/int T-cells. Reconstituted and MAP infected mice compared to mice with reconstitution only. No differences in expression of the indicated genes were found in samples without T-cell reconstitution (data not shown). The expression of the genes of interest was normalized against r18S RNA. Ctrl = <i>Rag2^−/−</i> mice treated with PBS only; Inf = <i>Rag2^−/−</i> mice infected with MAP; Rec = <i>Rag2^−/−</i> mice reconstituted with CD4⁺CD45RB^lo/int; Inf&Rec = <i>Rag2^−/−</i> mice infected with MAP and reconstituted with CD4⁺CD45RB^lo/int. n = 4. Bars depict median ± SEM. Statistical significance (p<0.05) is indicated by *.