<i>Drosophila</i> microbiota impacts midgut genes expression.

<p>(A) A schematic representation of the experimental set-up used for transcriptome analysis; GF: Germ-Free. (B) A volcano plot showing the absolute value of the computed statistics from the SAM analysis (d-stat) based on to the fold change in gene expression between the GF and poly-associated groups. The genes selected as differentially expressed by the SAM analysis are highlighted with a red circle around them. The selection was made using a value of Delta that corresponds to a FDR of 0.2. (C) Adult tissue enrichment classification of the 105 microbiota-regulated genes. (D) A bar graph representing the fold-enrichment of different microbiota-regulated transcripts in dissected midguts from GF vs poly-associated wild-type animals (the value of the relative ΔCt<i>^gene</i>/ΔCt<i>^rp49</i> ratio was calculated for every sample and relativized to the ratio in the GF condition which was anchored to 1 to indicate fold induction). Statistical significance of the result is represented (Student's t-test: ns≥0.05>*≥0.01>**≥0.001>***). Of note, <i>PGRP-SC1</i> mRNAs are detected to a 16 folds higher level than <i>PGRP-SC2</i> mRNA in young poly-associated animals (ΔCt<i>^PGRP-SC1</i>/ΔCt<i>^rp49</i> = 63 and ΔCt<i>^PGRP-SC2</i>/ΔCt<i>^rp49</i> = 4). (E) Relative percentage of Gene Ontology Terms: Biological processes in the list of microbiota-regulated gene (pie chart) and (F) Enrichment p-values (Benjamini-Hochberg corrected) for GO:Biological processes and GO:Molecular Functions in the list of microbiota-regulated genes.