Transcriptional profile of Arp2/3 complex (all subunits) in <i>D. variabilis</i> tissues.

<p><i>R. montanensis</i> was used to infect tick midgut, ovary, and salivary glands (8×10⁷ rickettsiae per tissue) for 1 h. After removal of rickettsiae, tick tissues were washed and collected by low-speed centrifugation. Total RNA was then extracted from the tissues and the levels of <i>Dv</i>Arp2/3 complex mRNA were measured by qRT-PCR. <i>Dv</i>GAPDH mRNA was used to normalize the differences among samples. Data shown are mean (±SEM) relative expression from two independent experiments. The asterisk denotes a significant difference between treatment groups (unexposed- or <i>Rickettsia-</i>exposed group) in the same tissue. For each subunit, different letters above bars represents significance differences between tissues.