Representative high resolution melting curves for pummelo SNPs.

<p>The left and the right panels were melting curves and derivative melting curves respectively. Red and green curves were homozygous amplicons with low and high melting temperatures, respectively. Gray and blue curves represent heterozygotes. Top panels: melting curves of an A/G SNP (chr1_20043485A/G). Middle panels: melting curves of an A/T SNP (chr1_25991020A/T). Bottom panels: melting curves of an A/G SNP (chr4_18094735A/G); note the two different heterozygotes with different melting curves, the normal heterozygote (referred as He1) as shown in gray and the abnormal heterozygote (referred to as He2 that probably contained a primer-template mismatch) in blue.