Embryonic and larvae zebrafish on microfluidic chip.

<p>(A) Schematic of chip for zebrafish assay. The chip includes two independent zones, each with a media inlet, a drug inlet, a gradient generator and seven series of fish tanks (one concentration with three tanks) named C1–C7. Left zone for embryonic toxicity and teratogenicity experiment, the right for larvae fish based drug toxicity evaluation. (B) Photos of the microfluidic chip and micrographs of the embryo and larvae in the chip.