Characterization of EPCs.

<p>Flow cytometric analysis of EPCs. (A) Percentage of expression of CD45, CD11b, CD90, CD44, CD31 and Endothelium markers were shown. Each column represents mean ± SD of six different clones. (B) mRNA levels of VEGF-A, ANG-1, PECAM-1 and HGF in cultured EPCs (***p<0.0001 VEGF-A versus ANG-1; ***p<0.0001 VEGF-A versus HGF; ***p<0.0001 ANG-1 versus PECAM-1; *p<0.05 ANG-1 versus HGF; ***p<0.0001 PECAM-1 versus HGF). Error bars are ± SEM. (C-F) In vitro functionality of EPCs was identified by positive staining for endothelial antibodies (Endothelium (C, green), Dil-Ac-LDL (D, red), nuclei (E, blue), merge (F)).(G, H) EPCs cobblestone-like morphology.