Role of UCH-L1 in the control of p21^WAF1/Cip1 (p21) protein expression in the presence of rapamycin in rat neonatal cardiac fibroblasts.

<p>Effect of UCH-L1 overexpression on PDGF-BB-induced mTOR phosphorylation and upregulation of p21^WAF1/Cip1 protein expression in the presence of rapamycin. Quiescent cells infected with Ad-control or Ad-UCH-L1 were pretreated with rapamycin (RAPA, 0.1 µM) or vehicle DMSO for 1 h, and followed with co-treatment of RAPA (0.1 µM) and PDGF-BB (20 ng/ml) for additional 24 h. Upper panel: representatives of immunoblotting. Lower panel: quantitatively densitometric analysis of protein expression. Data is presented as fold change of ratio of target protein to internal control β-actin relative to the Control (-) n = 4, *p<0.05. # p<0.05 vs. DMSO (-); & p<0.05 vs. DMSO (-) and PDGF-BB (+); @ p<0.05 vs. Ad-Controls. All results are representatives of at least 4 separated experiments.