MMP8 and MMP-9 expression in mice 3 weeks after common bile duct ligation (CBDL).

<p>(a) Immunoreactivity of MMP-9 (matrix metallopeptidase) is stronger than that in sham-operated mice; (b) Immunohistochemical analysis. Scale bar = 100 µm. (c) Immunoblotting of MMP-9 in the whole pulmonary tissue of the sham and of the 2 and 3 weeks after CBDL groups revealed that MMP-9 is highly expressed in the 2 and 3 weeks after CBDL groups, compared with the expression in the sham groups. (d) Immunoblotting of F4/80, CD31, and MMP9 in the CD31-positive pulmonary cell lysates obtained 3 weeks after CBDL showed higher levels of MMP9 expression in pulmonary vascular endothelial cells compared with those of CD31-negative pulmonary cells. (e) Immunoreactivity of MMP-8 is stronger than that in sham-operated mice; (f) Immunohistochemical analysis. Scale bar = 100 µm.