Hierarchical clustering analysis and qRT-PCR verification.

<p>(A) Hierarchical clustering analysis of all experimental samples. The reliable probes Horizontal stripes represent genes while columns show experimental samples (HP n = 3 and HR n = 3). Relationships were represented by the tree lines at the right side of the heat map (short line, close relation; long line, distant relation). (B) Heat map of the differentially expressed genes (DEGs) between the HP and HR groups. 1140 genes (<i>P</i><0.05, FC>1.5) were identified from the hierarchical cluster, including 602 upregulated genes and 538 downregulated genes. Gene expression was shown in the heatmap as up-regulated (red color), down-regulated (green color) and no change (black color). (C) Hierarchical clustering analysis of 10 DEGs (<i>P</i><0.05, FC>2). The genes (left) and probes (right) were shown in the heatmap. Gene expression profile was shown in the heatmap as up-regulated (red color), down-regulated (green color) and no change (black color). (D) The verification results of qRT-PCR. <i>Adcy8, Cytl1, Pcdhb6, Rgs1, Dpp4</i>, were up-regulated, <i>Bcl3, Kcnma1, Tnnt1, Mfsd10, Plk3</i> were down-regulated in the HP group compared with the HR group. Fold change was calculated based on the mean intensity value from 3 rats by using the comparative Ct method and normalized to the housekeeping gene <i>β-actin</i>, *<i>P</i><0.05, ns: no significant difference.