The involvement of lysosomes in E2-induced ERα degradation in MCF-7 cells.

<p>(A) XTT assay in growing MCF-7 cells treated for 24 hrs with different doses of chloroquine (Clo). (B) Western blot and relative densitometric analyses (C’) of ERα cellular levels in MCF-7 cells treated for 2 hrs with E2 (10 nM) in the presence of different concentrations of chloroquine (Clo). (C) Western blot and relative densitometric analyses (C’) of EGF-R cellular levels in HeLa cells treated for 2 hrs with EGF (1 µg/ml) in the presence of different concentrations of chloroquine (Clo). (D) Western blot and relative densitometric analyses of ERα cellular levels in MCF-7 cells treated with chloroquine (Clo 10 µM) in the presence of different doses of E2. (E–F) Western blot and relative densitometric analyses of ERα cellular levels in MCF-7 cells treated for with E2 (10 nM) at different time points in the presence of chloroquine (Clo 10 µM). Inhibitor alone was administrated for 2 hours and 30 min. Loading control was done by evaluating vinculin expression in the same filter. *indicates significant differences with respect to the control sample (0 or -); °indicates significant differences with respect to the corresponding E2-treated or EGF-treated samples (<i>p</i><0.05). Figure shows representative blots of at least three independent experiments.