Generation of <i>Pou4f1</i> and <i>Pou4f2</i> conditional knockout alleles.

<p>(A) Targeting strategy for generating <i>Pou4f1</i> conditional null allele. (B) Targeting strategy for generating <i>Pou4f2</i> conditional null allele. (C) Southern blot confirmed selection of <i>Pou4f1^cko</i> allele and PCR genotyping of <i>Pou4f1^loxP</i> heterozygous and homozygous. (D) Southern blot confirmed selection of <i>Pou4f2^cko</i> allele and PCR genotyping of <i>Pou4f2^loxP</i> heterozygous and homozygous. White boxes are the non-coding exon sequences and black boxes are the coding sequences. Thick bars are the sequences used to generate the homologous arms in the targeting vector. Restriction enzyme sites: A, <i>AvrII</i>; B, <i>BamHI</i>; E, <i>EcoRI</i>; H, <i>HindIII</i>; N, <i>NotI</i>; S, <i>SacII</i>; X, <i>XbaI</i>.