Enhancement of AAM polarization by IL-6 is not dependent on autocrine IL-10.

<p>(A–B) Macrophages derived from bone marrow of IL-10^-/- mice are still able to enhance arginase activity and suppress nitric oxide in response to IL-4+IL-13 + IL-6 stimulation (*p<0.05 compared to all groups, n = 3). (C–D) Addition of IL-10 neutralizing antibody (10 µg/ml) to the culture medium affected neither the enhanced arginase activity (C) nor the suppressed LPS (1 µg/ml) stimulated nitrite output (D) from wild-type murine bone-marrow derived AAMs differentiated in the presence of IL-6 (n = 3) (mean±SEM, * and # p<0.05 compared to macrophage (MØ) and AAM, respectively).