Endogenous CHRONO rhythmically binds to the E-box on circadian gene promoter.

<p>(A and B) ChIP analysis for CHRONO and IgG (negative control). The CHRONO occupancies at the endogenous E-boxes of <i>Per2</i> (A) and <i>Dbp</i> (B) promoters were detected in the two different NIH3T3 cells 32 or 44 h after induction with dexamethasone. The graphs showed relative real-time PCR values compared to input. Data are means ± S.E.M. of three samples (*<i>p</i><0.05, **<i>p</i><0.01, Student's <i>t</i> test). (C) ChIP abundance of CHRONO and BMAL1 at the <i>Per2</i> promoter E-box in NIH3T3 cells after 100 nM dexamethasone stimulation. The graph showed relative real-time PCR values. The data were plotted as percentages relative to the average (1.0). Data are means ± S.E.M. of 3–4 samples.