Endothelial turnover.

<p>Gene expression profiles of endothelial relevant genes were evaluated to analyze endothelial turnover. VE-cadherin, Tie-2 and KDR mRNA expression was analyzed using RT-PCR, PAI-1 and VEGF protein expression was analyzed using a protein array. PCR analyses were performed in triplicates and for protein analyses, pooled samples from each group was measured in duplicates. Columns and error bars represent means ± SEM; red line represents the time-course of PAI-1 and VEGF in wt animals and black line corresponds to the TLR-3 ko mice; ∫*, P<0.05, **, P<0.01 and ∫∫∫***, P<0.001; one-way ANOVA and Bonferroni's multiple comparison test. ƒ considered statistically different from the corresponding sham and * considered different from the corresponding wt time-point.