miR-338 decreases tumor growth and suppresses D-MVA by targeting NRP1 in vivo.

<p>(A) miR-338 expression in nude mouse tumors was measured using qRT-PCR. (B) Left panel: primary tumor growth after the orthotopic injection of 1×10⁶ AGS cells with the forced expression of miR-338 or cont-miR, with or without NRP1 restoration. Right panel: a representative image of xenograft tumors from nude mice. (C) NRP1 expression in nude mouse tumors was detected by western blot. (D) NRP1 expression in nude mouse tumors was detected by immunohistochemical staining. The arrows indicate NRP1. (E) CD34staining revealed few patent vessels per field in the tumors that overexpressed miR-338; however, the vessel number was restored in the tumors that overexpressed NRP1. (F) Binary images of CD34 staining with lumens digitally filled show lower D-MVA in miR-338 tumor sections and D-MVA was restored in the tumors that overexpressed NRP1. (G) The D-MVA was decreased in nude mouse tumors that overexpressed miR-338; however, the D-MVA was restored in the tumors that overexpressed NRP1. The data represent the means±s.d.; * p<0.01.