Effect of salinomycin and TRAIL added alone or in combination on caspase-8 activation and mitochondrial function.

<p>(A) Western blot analysis of cellular extracts derived from T98G and U251 cells incubated for 8 h and 24 h respectively, either in the absence (NT) or in the presence of salinomycin (10 µM), TRAIL (50 ng/ml) or salinomycin+TRAIL (at the above concentrations). The cell extracts were first run on SDS-PAGE, transferred to nitrocellulose membranes and blotted with either anti-human caspase-8 or anti beta-actin. One representative experiment out of three performed is shown. (B) Analysis of the mitochondrial membrane potential (Ψm) in T98G and U251 cells grown in the various conditions reported in the figure for 8 h and 24 h (T98G) or 48 h (U251). The proportion of cells exhibiting low Ψm is reported.