Evaluation of peptide-specific IL-2, IFNγ and TNFα expression in CD4 and CD8 T-cells.

<p>A representative example of the analysis performed in Vac1 (left panel) and Vac2 (right panel), two weeks after the final vaccine boost, is shown. PBMC were first expanded for a 12 day period using a pool of all NS3 peptides, restimulated with individual peptides (medium alone, p59 or p64) and analyzed for induction of IFNγ, IL-2 and TNFα cytokine expression by expanded CD4 and expanded CD8 cells, using the gating strategy described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095103#pone.0095103.s001" target="_blank">Figure S1</a>. The numbers in the quadrants, represent the percentage of positive cells calculated from the parent-population.