Airway constriction changes the subcellular structures of actin and microtubule.

<p>Single and double-labeled immunohistochemistry showing the distribution of β-actin, ASMA, the polymerized actin (F-actin) and α-tubulin on representative sections of bovine intact airways after static and dynamic Ptm conditions (90 min mechanical loading) and with constriction (<b>A</b>) and actin or microtubulin depolymerization (<b>B</b>). β-actin and F-actin are shown in brown; ASMA and α-tubulin are blue and nuclei are pink (<b>A</b>, and <b>B</b>). All conditions were processed simultaneously for each antibody (n = 30/condition). Scale bars are 100 µm on the top row sections and 50 µm on the bottom rows.