ADF/Cofilin gene silencing induces spine loss and shrinkage.

<p><i>Upper Panel</i>: Images from selected dendritic regions of neurons expressing the eGFP-tagged pSuper empty vector, the scrambled control, or the cofilin/ADF-RNAi (cof/ADF-RNAi) for 4 days prior to fixation. Image width = 27.3 µm. <i>Lower Panel</i>: Quantification of dendritic spine density, length and width. Data are expressed as mean ± SEM; ***p<0.001, Kruskal-Wallis test followed by Dunn's multiple comparisons test, number of dendrites: empty v. = 34, scramble = 13, cof/ADF-RNAi = 61; number of spines: empty v. = 642, scramble = 251, cof/ADF-RNAi = 712. Experiments in this figure and its corresponding supplemental material were analyzed in a blind fashion.