Kinetic parameters of <i>Ac</i>CR for reduction of 4′-chloroacetophenone and oxidation of isopropanol.

<p>Kinetic constants were spectrophotometrically analyzed by determining the initial reaction rate at 340 nm at different substrate concentrations. Reactions were carried out in the optimal conditions. The dependence of <i>Ac</i>CR's activity on substrate concentrations and the corresponding Michaelis–Menten curve or Hill curve were illustrated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094543#pone.0094543.s002" target="_blank">Figures S2</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094543#pone.0094543.s005" target="_blank">5</a>.</p>a<p>Determination for the NAD(H)-linked reaction.</p>b<p>Determination for the NADP(H)-linked reaction.</p>c<p>The apparent K_m value was considered as S_0.5.