KO Tregs were hyperfunctional.

<p>CFSE-labeled Teffs were stimulated in the presence of DCs and Tregs from WT or KO mice. On the 3^rd day, the cells were harvested and stained for surface CD4. Live CD4⁺CFSE⁺ cells were gated for the analysis of the proliferative responsiveness of Teffs. The proliferative response of Teffs in the presence of KO Tregs was less active than in the presence of WT Tregs, suggesting KO Tregs were hyperfunctional in the suppression of Teffs than WT Tregs. KO, GPx1^−/− × Cat^−/−. Data are mean ± SE of six separate experiments.