Intracellular ROS level was higher in GPx1^−/−× Cat^−/− than WT lymphocytes.

<p>Splenocytes were stained with DC-FDA (5 µM) and simultaneously with anti-CD4-PerCP and anti-CD25-PE (A) or anti-CD11c-PE (B). Then the cells were washed and acquired using FACSCalibur. After stabilization for 30 sec, acquisition was briefly suspended and PMA (100 nM) was added to the tube, and the cells were acquired further for 482 sec. ROS level was expressed as mean fluorescence intensity (MFI) of DC-FDA fluorescence. Data are mean ± SE (n = 12). *, P<0.05.