<p>pGL3-300, pRL-TK, and Sp1 siRNA or non-targeted control siRNA were co-transfected into LS-174T cells and cultured for 48 h. A. Relative luciferase activity (*P<0.05 <i>vs</i>. SiControl). B. Detection of <i>Sp1</i> and <i>hTFF3</i> mRNA levels by real-time PCR (*P<0.05 <i>vs</i>. SiControl). C and D. Detection of hTFF3 protein by western blotting.</p
Specificity protein 1 (Sp1) is an important transcription factor implicated in numerous cellular pro...
<p>(<b>A</b>) Reporter assay using constructs including 3 copies of 31 bp DNA fragment flanking SNP ...
<p>(A) <i>Hsf1.2<sup>−/−</sup></i> iMEFs were co-transfected with 12.5 ng of pCR3.1-HSF1α or pCR3.1-...
<p>Both pGL3-300 and a Sp1 over-expression plasmid were co-transfected into LS-174T cells and cultur...
<p>A. ChIP assay: LS174T cells were fixed with formalin, and then the chromatin was cleaved by sonic...
<p>(<b>A</b>) RNAi knockdown against <i>HSF1</i>. Two siRNAs directed against <i>HSF1</i> (siHSF1_#1...
<p>(A) Schematic showing luciferase constructs with different lengths of the <i>CD3ζ</i> promoter. (...
<p>293T cells maintained in 24-well plates were co-transfected with (i) 200 ng DNA of a pGL3-Basic l...
The transcription factor Sp1 plays a key role in the activation of many cellular and viral gene prom...
<p>Transcriptional activation of the hTERT gene by FOXO3a was determined by normalized luciferase ac...
<p><b>A.</b> Luciferase reporter constructs (in pGL-3 basic vector) carrying upstream sequences of 2...
<p>(A) HIF-1α activates the <i>Sost</i> promoter in a dose-dependent manner. HEK293 cells were trans...
<p>Luciferase activity was normalized to control plasmid (pRL-CMV) expression. The results are shown...
<p>(<i>A</i>) The effect of HTRA1 on TGF-β transcription was examined by co-transfecting HeLa cells ...
<p>(A) <i>Hsf1.2<sup>−/−</sup></i> iMEFs were co-transfected with increasing quantity of pCR3.1-HSF1...
Specificity protein 1 (Sp1) is an important transcription factor implicated in numerous cellular pro...
<p>(<b>A</b>) Reporter assay using constructs including 3 copies of 31 bp DNA fragment flanking SNP ...
<p>(A) <i>Hsf1.2<sup>−/−</sup></i> iMEFs were co-transfected with 12.5 ng of pCR3.1-HSF1α or pCR3.1-...
<p>Both pGL3-300 and a Sp1 over-expression plasmid were co-transfected into LS-174T cells and cultur...
<p>A. ChIP assay: LS174T cells were fixed with formalin, and then the chromatin was cleaved by sonic...
<p>(<b>A</b>) RNAi knockdown against <i>HSF1</i>. Two siRNAs directed against <i>HSF1</i> (siHSF1_#1...
<p>(A) Schematic showing luciferase constructs with different lengths of the <i>CD3ζ</i> promoter. (...
<p>293T cells maintained in 24-well plates were co-transfected with (i) 200 ng DNA of a pGL3-Basic l...
The transcription factor Sp1 plays a key role in the activation of many cellular and viral gene prom...
<p>Transcriptional activation of the hTERT gene by FOXO3a was determined by normalized luciferase ac...
<p><b>A.</b> Luciferase reporter constructs (in pGL-3 basic vector) carrying upstream sequences of 2...
<p>(A) HIF-1α activates the <i>Sost</i> promoter in a dose-dependent manner. HEK293 cells were trans...
<p>Luciferase activity was normalized to control plasmid (pRL-CMV) expression. The results are shown...
<p>(<i>A</i>) The effect of HTRA1 on TGF-β transcription was examined by co-transfecting HeLa cells ...
<p>(A) <i>Hsf1.2<sup>−/−</sup></i> iMEFs were co-transfected with increasing quantity of pCR3.1-HSF1...
Specificity protein 1 (Sp1) is an important transcription factor implicated in numerous cellular pro...
<p>(<b>A</b>) Reporter assay using constructs including 3 copies of 31 bp DNA fragment flanking SNP ...
<p>(A) <i>Hsf1.2<sup>−/−</sup></i> iMEFs were co-transfected with 12.5 ng of pCR3.1-HSF1α or pCR3.1-...