Effect of TLR7 knockdown on imiquimod-induced apoptosis of SCC12 cells.

<p>(A) Cells were transduced with 10 multiplicity of infection (MOI) of adenovirus expressing miR-TLR7 or control adenovirus (miR-Scr) for 6 h. Cells were replenished with fresh medium, and incubated for a further 2 d. Endogenous expression of TLR7 is markedly decreased by miR-TLR7. (B) After adenoviral transduction, cells were treated with imiquimod (150 µg/ml) for 16 h and apoptosis was determined by flow cytometry. Bold boxes represent apoptotic cells. There is no difference in apoptotic cell populations between TLR7-knockdowned group (miR-TLR7) and control group (miR-Scr). (C) Cleavages of PARP was detected by Western blot. TLR7 knockdown does not affect imiquimod-driven PARP cleavage.