<i>TnBVank1</i> expression causes the block of the transition from larval to pupal stage.

<p>(A) Light micrographs of <i>yw;h-Gal4</i> larva and pupa (control) and <i>h-Gal4>TnBVank1</i> larvae at different days AED. The scale bar is 500 µm. (B) Larval length of different genotypes, at 96 h AED. Five larvae of each genotype were analyzed and as control we measured larval length of <i>yw</i> and <i>h-Gal4</i> and <i>UASp-TnBVank1</i> stocks and <i>yw;h-Gal4</i>. Graph represents mean ± standard deviation (s.d.); there is no significant (NS) length difference between <i>h-Gal4>TnBVank1</i> (2680±83 µm) and <i>yw;h-Gal4</i> larvae (2580±82 µm). (C) Larval length of <i>h-Gal4>TnBVank1</i> increases during the extended larval life. Five <i>h-Gal4>TnBVank1</i> larvae were measured at different days AED; values are the mean ± s.d. of three independent experiments. The mean values of <i>h-Gal4>TnBVank1</i> larval length at four and eighteen days AED are shown above the bars.