RNG2 is required for invasion, motility and rhoptry evacuole formation.

<p>(A) Percentage parasite invasion success of iΔHA-RNG2 cells grown either with or without ATc. >200 parasites were scored for each of three biological replicates. (B) Gliding motility detected by SAG1-positive trails of iΔHA-RNG2 cells with or without ATc. Motility was also assessed with further treatments of actin polymerization inhibitor cytochalasin D, or calcium ionophore A23187. (C) Percentage of ROP1-positive evacuoles formed by iΔHA-RNG2 cells grown with or without ATc. >200 parasites were scored for each of six biological replicates. Error bars = standard error of the mean, * denotes significant differences (P<0.05, two-tailed Student's t-test).