Representative results of <i>DACH1</i> methylation and expression in primary esophageal cancer.

<p>(A) Representative MSP results of <i>DACH1</i> methylation status in normal esophageal mucosa (NE), esophageal dysplasia (ED) and esophageal cancer (EC). (B) <i>DACH1</i> methylation frequency in NE, ED1, ED2 and ED3, and EC. The frequency of methylated <i>DACH1</i> were plotted according to histological grade and analyzed using chi-square test. **, <i>P</i><0.01. (C) Representative IHC results for DACH1 expression in primary esophageal cancer (left) and adjacent tissues (right); upper phase, X200; lower phase, X400. (D) DACH1 expression level in 30 cases matched primary cancer and adjacent tissue samples; box plot: represents DACH1 expression level; horizontal line: represent the median level; the top and bottom line of the boxes represent 75% and 25% expression level, respectively; vertical bars represent different expression level. **, <i>P</i><0.01 versus adjacent tissue samples by using Wilcoxon signed-rank test. (E) The association of <i>DACH1</i> methylation and loss/reduced expression in 30 cases ESCC. **, <i>P</i><0.01, Spearman’s rank correlation coefficient.