Analysis of <i>pds2-1</i> mutant plants.

<p>(<b>A</b>) WT and homozygous <i>pds2-1</i> (T₃) seedlings with purple cotyledons grown on ½ strength MS medium for 4 days. (<b>B</b>) Adult homozygous <i>pds2-1</i> mutant (T₃) and WT plants grown on ½-strength MS medium for 4 weeks. (<b>C</b>) A purple T₃<i>pds2-1</i> mutant seedling. Scale bar = 1 mm. (<b>D</b>) Inflorescences and flower buds from 8-week-old WT and <i>pds2-1</i> plants. (<b>E</b>) Roots from <i>pds2-1</i> and WT plants. (<b>F</b>) Adult <i>pds2-1</i> plant grown on ½ strength MS medium for 8 weeks. Scale bar = 2 mm. (<b>G</b>) Adult inflorescence of a <i>pds2-1</i> mutant plant. Scale bar = 0.5 mm. (<b>H</b>) <i>HST</i> gene structure and the T-DNA insertion site in <i>pds2-1</i> mutants. Black boxes: <i>HST</i> gene exons; black lines: <i>HST</i> gene introns. (<b>I</b>) Co-segregation analyses of the HST transgene with <i>pds2-1</i> mutant phenotypes. Marker: DNA marker; <i>PDS2/pds2</i>: heterozygous <i>pds2-1</i> mutants; <i>pds2/pds2</i>: homozygous <i>pds2-1</i> mutants. (<b>J</b>) RT-PCR analysis of the <i>HST</i> gene in WT and <i>pds2-1</i> mutant plants. The <i>UBQ10</i> gene was used as an internal control.