LY294002 partly inhibited protective effect of dexmedetomidine (Dex) pretreatment in the hippocampus of P7 rats (n = 8 in each group).

<p>(A) Representative Western blot of cleaved caspase-3, phospho-Akt, Akt, phosho-Bad, Bad and Bcl-xL; (B, C, D, E, F) The quantitative analysis of cleaved caspase-3 (B), ratio of phospho-Akt to Akt (C), ratio of phospho-Bad to Bad (D), ratio of Bad to β-actin (E) and ratio of Bcl-xL to Bad (F) by one-way ANOVA and post-hoc Bonferronni's correction. Results are the means ± SD. NS: normal saline, DMSO: dimethyl sulfoxide, LY: LY294002, Dex75: Dex 75 µg/kg, Iso: isoflurane. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 <i>versus</i> Air+NS; ^#<i>P</i><0.05, ^##<i>P</i><0.01, ^###<i>P</i><0.001 <i>versus</i> Iso+NS; ^▴▴<i>P</i><0.01, ^▴▴▴<i>P</i><0.001 <i>versus</i> Iso+Dex75.