PF13_0159 encodes a nicotinate mononucleotide adenylyltransferase.

<p><b>A</b>. Dependence of PF13_0159 adenylyltransferase activity on time and enzyme concentration. A discontinuous assay was established to measure enzyme activity; pyrophosphate release is measured as the end product of the adenylyltransferase reaction. Values were normalized to background absorbance values obtained in a buffer only control. A standard curve was generated to determine pyrophosphate concentrations. Error is reported at the SD of three independent technical replicates. <b>B</b>. Phylogenic analysis of PF13_0159 compared to representative prokaryotic and eukaryotic NMNATs. ClustalW2 was used to generate alignments and distance values are reported. An alignment of PF13_0159 and the <i>E. coli</i> NADD is shown in Figure S5 (in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094061#pone.0094061.s001" target="_blank">File S1</a>).