Amoebae harboring <i>Neochlamydia</i> S13 are completely protected from <i>L. pneumophila</i> infection.
- Kasumi Ishida (281240)
- Tsuyoshi Sekizuka (249989)
- Kyoko Hayashida (281242)
- Junji Matsuo (187334)
- Fumihiko Takeuchi (118220)
- Makoto Kuroda (249974)
- Shinji Nakamura (187336)
- Tomohiro Yamazaki (281239)
- Mitsutaka Yoshida (187344)
- Kaori Takahashi (187346)
- Hiroki Nagai (236046)
- Chihiro Sugimoto (134113)
- Hiroyuki Yamaguchi (187348)
Publication date
April 2014
DOIAbstract
<p>(A) <i>L. pneumophila</i> survival in <i>Neochlamydia</i> S13-infected amoebae (S13WT) and aposymbiotic amoebae (S13RFP). (B) <i>L. pneumophila</i> growth in <i>Protochlamydia</i> R18-infected amoebae (R18WT) and aposymbiotic amoebae (R18DOX). (C) <i>L. pneumophila</i> survival in C3 reference strain amoebae (C3), and PYG medium only (Med). Data are the means ± SD from at least three independent experiments performed in triplicate. * denotes <i>p</i><0.05 at each of the time points. (D) Representative Gimenez staining images showing <i>L. pneumophila</i> replication into amoebae at 5 days post-infection. Arrows, <i>Neochlamydia</i> S13 (S13WT) or <i>Protochlamydia</i> R18 (R18WT). Arrowheads, replicated <i>L. pneumophila</i>.</p
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