Upregulation of legumain expression in brainstem neurons at 11 days after SCI.

<p>(A) Double staining of <i>legumain</i> mRNA (<i>in situ</i> hybridization) and NeuN (immunohistochemistry) was performed to determine the identity of <i>legumain</i>-positive cells. The signal for NeuN locates in the nucleus and <i>legumain</i> mRNA locates in the neuronal cytoplasm in the NMLF and IMRF. (B) Immunohistochemistry for Legumain shows more Legumain positive neurons (arrows) in the NMLF and IMRF at 11 days after SCI when compared to the sham-injured control. Dorsal is up. A, <i>n</i> = 3 experiments; B, <i>n</i> = 3 fish. Scale bar, 50 µm.