<i>Legumain</i> mRNA expression is upregulated in brainstem neurons during the axonal regrowth phase after SCI.

<p>(A) <i>In situ</i> hybridization was performed to study the expression of <i>legumain</i> in the NMLF and IMRF. Representative images depict <i>legumain</i>-positive cells in the NMLF and IMRF 11 days after SCI. More positive cells for <i>legumain</i> mRNA are observed in the NMLF and IMRF after SCI when compared with sham-injured control. With the sense control probe, no signal is observed. (B, C) Quantification shows that <i>legumain</i> mRNA expression is slightly upregulated in the NMLF and IMRF at 3 days and highly upregulated at 11 days after SCI. The expression patterns of <i>legumain</i> in the NMLF and IMRF are similar. Dorsal is up. NMLF, <i>n</i> = 6 fish; IMRF, <i>n</i> = 3 fish. * <i>P</i><0.05, two-tailed <i>t</i>-test; mean values ±SEM are shown. Scale bar, 50 µm.