Tonic EPSPs are inhibited using BoNT/B-LC under low frequency stimulation.

<p><b><i>A</i></b>, Example showing the tonic action potentials (APs, 200 Hz) remain unchanged before (<i>i</i>) and after (<i>ii</i>) the injection of each neurotoxin (BoNT/B-LC used as the example). TeNT-LC (<i>B</i>) and BoNT/D-LC (<i>C</i>) did not have an effect on the tonic evoked response. <b><i>E</i></b>, Percent difference between active and inactive neurotoxin, in which the inactive neurotoxin is the reference point at 0%. In <i>B-E</i>, a solid black line represents the time course of neurotoxin injection (90 min) and vertical arrows (↓) represent when EPSPs were recorded. Active neurotoxins: TeNT-LC (▪), BoNT/D-LC (▴) and BoNT/B-LC (•). Inactive neurotoxins: TeNT-LC (□), BoNT/D-LC (△) and BoNT/B-LC (○). Error bars represent S.E.M. The measured tonic (last) EPSP is encircled in black (<i>Ai</i>). An asterisk (*) denotes a significant difference (p<0.05) and ‘n’ represents the sample size (active/inactive neurotoxin). Scale bars: horizontal – 10 ms; vertical – 10 mV (APs), 5 mV (EPSPs).