The RCO-1/RCM-1 complex is required for the accumulation of VVD after exposure to light.

<p>(A) The RCO-1/RCM-1 complex is required for the light-dependent mRNA accumulation of <i>vvd</i>. Quantitative RT-PCR experiments were performed to measure the relative accumulation of mRNA in mycelia of the wild type, a strain with a deletion of <i>rco-1</i> or a strain with the <i>rcm-1^RIP</i> allele exposed to white light (1 W/m² blue light) or kept in the dark. The plots show the average and standard error of the mean of the relative mRNA accumulation in three independent experiments. The results from each PCR for each gene were normalized to the corresponding PCR for <i>tub-2</i> to correct for sampling errors and normalized to those obtained with the wild type after exposure to 30 min of light. (B) A reduction in the amount of VVD in the Δ<i>rco-1</i> strain. Strains were ground on Vogel's minimal medium for 48 hours at 22°C in the darkness or under white light (11 W/m²). Then, samples grown in the dark were exposed for 30 minutes to light. Total protein extracts isolated from mycelia of the wild-type strain were separated by SDS–PAGE, and hybridized with anti-WC-1, anti-WC-2 and anti-VVD antibodies. 200 µg of proteins were loaded per lane. The bands that correspond to each protein are marked by an arrow, additional bands are due to WC-2 modification or to the non-specific binding of the WC-1 antibody. The experiment was repeated three times and a representative protein hybridization is shown.