Rest binding sites that affect transcription.

<p>(A) Rest knock-down analysis. We show the indicated gene mRNA expression levels measured using RT-qPCR and the Rest results from two independent qPCR primers. (B) The ES-unique (left) and common (right) Rest binding sites distributions that show the indicated fold change in transcription. Populations with greater than a 2-fold increase in transcription are indicated with arrows. (C) The Gabrb3 Rest binding and transcript levels in ES and EpiS cells are compared. Gabrb3 induction through Rest knock-down and the Gabrb3 regulatory network model are also shown. (D) Rest and Sin3A ChIP Seq tags in the indicated cells are shown. Signal intensities of the active chromatin modifications (H3K4me3, RNA Pol2, and H3ac) surrounding Gabrb3 in ES and EpiS cells. (E) The Igfbp6 and Gli1 Rest binding and transcript levels in ES and EpiS cells are compared. The Rest binding sites around Igfbp6 and Gli1 are located in 50 kbp upstream and 80 kbp downstream of each genes, respectively. The Igfbp6 and Gli1 expression change due to Rest knock-down and the Igf2 regulatory network model are also shown.