Western Blotting analysis.

<p>The expression of some proteins measured at different treatment times, and involved in apoptotic signaling and/or cell cycle control, regulated by specific treatments. A total of 40 mg of whole cell lysate from HosDXR150 treated with 5-Aza-2-deoxicytidine (DAC) or Trychostatin A (TSA) or combined treatment (DAC+TSA) at different times (24, 36, 48, 72 and 96 hours) was electrophoretically fractioned in SDS-PAGE. The levels of protein expression increased beginning at 36 hours for RBL2, CASP10 and FAS, or at 48 hours for TP73, TP53 and IL12A, and reached a maximum at 96 hours. Beta-actin was used to normalize.