miR-17-5p modulates BECN1 expression in A549-T24 cells.

<p>(A) A549-T24 cells were transfected either with 100 nM pre-miR-negative control (T24-miR-NC) or pre-miR-17-5p (T24-miR-17-5p) precursor RNA. After 24 h, cell lysates were prepared for Western blotting with antibody against BECN1, MAP-LC3, p62 and GAPDH was used as loading control. (B–C) Relative BECN1 and LC3-II mRNA expression levels were quantified by qRT-PCR analysis in T24-miR-NC and T24-miR-17-5p cells, <i>bars</i> represent mean ± S.E. from three independent experiments (**p<0.01 vs control, where n = 3).