Dye fluorescence spectrum collected with Thioflavin T (ThT) alone (black) and with ThT in the presence of β2m aggregates (red) for aggregates such as those imaged in Figure 5.

<p>A profound increase in the emission of ThT is seen to peak at 482 nm (note the break in the y-axis) upon its binding to the cross beta-sheet structure in the aggregates. With amorphous aggregates, no such increase was seen and fluorescence was similar to that seen in control solutions of the dye.