Effects of <i>Trim30</i> deficiency on classical TCR signaling.

<p>(<b>A</b>) Primary CD4⁺ T cells from <i>Trim30</i>^+/+and <i>Trim30</i>^−/−mice were stimulated with the indicated antibodies for 3 days, and then the supernatants were collected and assayed for IL-2 production by ELISA. Concentration of IL-2 is shown for three independent experiments. Error bars represent s.d. (<b>B</b>) The numbers of CD25− and CD69− expressing CD4⁺ T cells from <i>Trim30</i>^+/+ (gray area) and <i>Trim30</i>^−/− (white area) mice 24 h after treatment with anti-CD3 (2 µg/ml) antibody are shown. Mean fluorescence intensity (MFI) values are given. (<b>C</b>) Purified CD4⁺ T cells from <i>Trim30</i>^+/+ and <i>Trim30</i>^−/− mouse spleens were stimulated with anti-CD3 antibody (2 µg/ml) and anti-IgG (10 µg/ml) for the times indicated at the top. Lysates from CD4⁺ T cells were analyzed by immunoblotting using the indicated antibodies. The bar graph shows the averages of the band intensities. Data are representative results of at least three independent experiments. Error bars in A and C denote the s.d.