Pyruvate induced DAPK1 dissociation from NMDA receptor and association with Beclin-1.

<p>Cells were pretreated with vehicle or 20°C. After preincubation, cells were challenged with vehicle or 30 mM glutamate for 3 hours. After treatment cells were lysed and the lysate was immunoprecipitated by anti-DAPK1 antibody. Then the immunoprecipitated proteins were detected with anti-NMDA receptor antibody (A) or anti-Beclin-1 antibody (B). All the immunoprecipitated protein was determined by anti-DAPK1 antibody (IP). The protein levels that were pulled down by DAPK1 in control cells were set as 100%. (C), Cell survival was determined by MTT assay. Cells were treated with vehicle, pyruvate for 30 minutes or preincubation with 100 nM DAPK1 inhibitor for 1 hour followed by 30 minutes pyruvate exposure. Then cells were challenged with 30 mM glutamate for 3 hours. All cells were cultured at 37°C. Cell survival of control cells was set as 100%. *<i>P</i><0.05 vs control; ^#<i>P</i><0.05 vs glutamate; n = 3.