Expression patterns of EBI3, p35, and IL-12Rβ2 in testes.

<p>(A) Cryostat sections of testes of WT mice (2 and 12 weeks old; <i>n</i> = 5 per group) were immunohistochemically stained with anti-EBI3, anti-CD163, anti-p35, anti-F4/80, anti-GATA1, anti-IL-12Rβ2, and DAPI. Representative confocal merged images are shown. F4/80, CD163, and GATA1 are specific markers for whole macrophages, a subset of resident macrophages and Sertoli cells, respectively. Similar results were obtained in more than two independent experiments. (B) Cryostat sections of testes of WT mice (12 weeks old; <i>n</i> = 3 per group) were immunohistochemically stained with anti-EBI3, anti-p35, anti-CD163, anti-Foxp3, and DAPI. Representative confocal merged images are shown. (C) The number of positively stained cells was counted in multiple fields of microscope and calculated as cells per field. Data are shown as mean ± SD. *<i>P</i><0.05 compared with Foxp3⁺ cells.