IKAP protein levels are highly reduced in FD mutant mice and after lentiviral ShIKAP administration in Oli-neu cells.

<p>(<b>A</b>) Western blot analyses of total protein lysates from brain cortex of control (N1, N2, N3) and age-matched FD (FD1, FD2, FD3) littermates. Upper panel shows detection of IKAP with the polyclonal anti-IKAP antibody (AnaSpec), and lower panel shows anti-β-actin for loading control. Note that IKAP protein expression is highly reduced in FD1, FD2, and FD3, relative to controls. Quantitative analysis of the Western blot was performed using Image J software. IKAP expression levels over β-actin levels are presented. (<b>B</b>) Western blot analyses of total protein lysates from control scrambled (ShSCR) and <i>IKAP</i> knockdown (ShIKAP) Oli-neu cells. Upper panel shows detection of IKAP with the polyclonal anti-IKAP antibody (AnaSpec), and lower panel shows anti-β-actin for loading control. Note that IKAP is significantly expressed in ShSCR Oli-neu cells. Quantitative analysis of the Western blot was performed using Image J software. IKAP expression levels over β-actin levels and are presented.