Inhibition of cell growth, invasion and migration by siRNA RET/PTC3.

<p>siRNA RET/PTC3 was tested for cell viability (<b>A</b>), LDH release (<b>B</b>), invasion (<b>C</b>) and migration (<b>D</b>) in RP3 cells. siRNAs (RET/PTC3, RET/PTC1 or Control) were transfected with Lipofectamine at 50 nM concentration in RP3 cells. (<b>A</b>) Cell viability was performed by MTT assay and (<b>B</b>) LDH release by LDH toxicology assay kit as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095964#s2" target="_blank">Materials and Methods</a> section. Measurements are performed at 24 h, 48 h and 72 h post-transfection. Monolayer RP3 cells was scratched and monitored every 4 h for invasion (<b>C</b>) or migration (<b>D</b>) assays by IncuCyte. Data were analysed by ANOVA followed by LSD Post-hoc test and presented as relative expressions in treated compared to non-treated cells. Stars represent the significant difference between the treatment groups compared to non-treated cells. * = <i>p</i><0.05, ** = <i>p</i><0.01, *** = <i>p</i><0.001.