Study design and workflow for the lipidomic analyses.

<p>Heterozygous female <i>fat-1</i> and WT mice were fed a 6-month-long diet containing 10% corn oil, which is particularly enriched in omega-6 PUFAs. Blood was collected and plasma samples were prepared and divided into two aliquots. Before extraction, a mixture of internal standards was added to the plasma to normalize for variations in sample preparation or MS detection. Complementary untargeted and targeted lipidomic analyses were conducted, and the results were integrated for the generation of a unique lipidomic biosignature characteristic of the balanced omega-6/omega-3 ratio found in <i>fat-1</i> mice.