Loss of <i>flcn-1</i> activates autophagy resulting in oxidative stress resistance in <i>C. elegans</i>.

<p>(A and B) Representative electron micrographs from longitudinal sections of the hypodermis in indicated nematodes strains. Arrows represent autophagic vacuoles (A) and autophagosomal membranes (B). Scale bars: 0.2 µm (A and B). (C) Quantification of the autophagic events observed in defined surface area of 4.25 um² of electron micrographs taken from at least 5 animals. Red lines represent the mean of autophagosome numbers per area indicated strains and treatment condition. (D) Number of GFP::LGG-1 positive autophagosome puncta in the seam cells of the indicated worm strains. (E) Western blot analysis of the GFP::LGG-1 cleavage profile (LGG-1-II, GFP) in worm protein extracts. (F, G, H, I). Percent survival of indicated strains upon 4 mM (F and H) or 100 mM (G and I) PQ. Data represent the means ± SEM, n≥3. Also see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004273#pgen.1004273.s011" target="_blank">Tables S2</a> and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004273#pgen.1004273.s012" target="_blank">S3</a>.