Fluorescence spectra of NBD-PS in LUVs in the presence of different molar ratios of total lipid to protein (C2α domain) at different lipid compositions.

<p>The molar ratio of total lipid to protein are indicated. (A) POPC/POPS/NBD-PS (molar ratio 80/19.5/0.5), (B) POPC/POPS/PIP2/NBD-PS in molar ratio 75∶19.5∶5∶0.5. (C) POPC/NBD-PC (molar ratio 99.5/0.5). A Ca²⁺ concentration of 100 µM was present in the assays, but control samples to which 3 mM EGTA was added instead of Ca²⁺ are shown.